Applications
FILM Core Facility Microscope
Dr. Martin Spitaler, Professor Tony Magee- Imperial College London
Situated in the Sir Alexander Fleming Building South Kensington, this system has been designed to meet a wide range of imaging requirements. Users are looking at everything from bacteria, mamalian celles, through to whole organism imaging, such as Zebra Fish. Ease of use and the technical ability of our staff was a prime consideration in the selection process.
The system also boasts an ASI XY stage with Piezo Insert, and can acquire up to 15 Z sections per second!
Future studies will include Calcium imaging and FRET.
News!! On July 4th we will be attending the official opening of this prestigious facility.
System Configuration
SPCI Image Capture Software
DIA (Dynamic Intensity Analysis) Ratiometric Software
Hamamatsu 9100-02 Camera
ASI XY Stage Piezo Z-Scanner
Live Cell 2.0 Environmental Chamber
Deconvolution Software
Long term imaging of cell division in mammalian cells.
Dr. Sally Wheatley- University of Sussex
Her laboratory is interested in cell division and the role of various proteins in mitosis and cytokinesis.
Typically they use cultured human cells (HeLa, U2OS and Hct116) and a combination of green
fluorescent and red fluorescent tagged cDNA constructs to localise our proteins of interest during
cell division. Experiments typicall record Multiple Position Time Lapse data over 3-7 days using our system.
System Configuration
SPCI Image Capture Software
Hamamatsu ORCA Camera
Leica DMIRB microscope
Live Cell 2.0 Environmental Chamber
Prior Motorised Stage and Illumination Control
High Speed Imaging of Platelets
Dr. Richard Farndale- University of Cambridge
Dr. Farndale is imaging fluorecently labelled platelets, and is studying ways of combating thrombus formation. Digital Pixel recommended the use of a high speed Hamamatsu 9100-02 camera system, combined with an Optical Beamplitter and PIFOC Z-Scanner. This system will allow the acquisiton of 2 wavelengths in Real Time at the frame rate of the camera- in excess of 25fps
System Configuration
SPCI Image Capture Software
DIA (Dynamic Intensity Analysis) Ratiometric Software
Hamamatsu 9100-02 Camera
PIFOC Piezo Z-Scanner
Protein-Protein Interactions in Rhodobacter sphaeroides
Armitage Group University of Oxford
Localisation of chemotaxis proteins in the bacterium Rhodobacter sphaeroides...More
